Genetically Engineered Tomato Plant Grows in Salty Water

DAVIS, California, August 9, 2001 (ENS) - Plant biologists have developed the first truly salt tolerant crop - a genetically engineered tomato plant that thrives in salty irrigation water and may hold the key to one of agriculture's greatest dilemmas. The tomatoes offer hope that other crops can also be genetically modified for planting in many areas of the world that have salty irrigation water and salt damaged soils.

"Since environmental stress due to salinity is one of the most serious factors limiting the productivity of crops, this innovation will have significant implications for agriculture worldwide," said Eduardo Blumwald, who led the research team that discovered the salt tolerance gene. The research, much of which was done at the University of Toronto, continues in the University of California - Davis Department of Pomology. Pomology is the science of fruit and nut production and distribution.

The most recent findings by Blumwald and Hong-Xia Zhang, a postdoctoral fellow at the University of Toronto, appear in the August issue of the journal "Nature Biotechnology."

Worldwide, an estimated 24.7 million acres (10 million hectares) of once agriculturally productive land are being lost each year because of irrigation induced salinity, according to the U.S. Department of Agriculture.

Crop production is limited by salinity on 40 percent of the world's irrigated land and on 25 percent of irrigated land in the United States.

This progressive loss of farmable land is on a collision course with the expanding global population, which over the next 30 years is expected to require an increase in food production of 20 percent in developed countries and 60 percent in developing nations.

Crop irrigation allows farmers to be less dependent on seasonal rainfall and the uncertainties of the weather. However, irrigation also increases the salinity of soils and water by depositing in the fields soluble salts such as sodium, calcium, magnesium, potassium, sulfate and chloride that the water has picked up from the soils and rocks it has passed through.

Eventually these salts accumulate in the irrigated soils at levels that decrease the productivity of the crops grown there.

tomatoes

Tomatoes from control plants grown at low salinity (left) and transgenic plants grown in the presence of 200 mM sodium chloride, also known as table salt (right). (Photo courtesy University of Toronto)
Salty irrigation water harms most plants by upsetting their ability to take in water through their root cells. If salt concentrations in the soil are very high, the flow of water into the plant is reversed and the plant dehydrates and dies as water is drawn out of its cells.

Although scientists have been trying to develop salt tolerant crop varieties using selective breeding techniques throughout the past century, none of those efforts has proven successful.

Blumwald and Zhang have genetically engineered tomato plants that produce higher levels of a naturally occurring protein known as a transport protein. The gene that controls increased production of the transport protein was taken from Arabidopsis, a relative of the cabbage that is commonly used in plant research.

The transport protein uses energy available in the cells to move salt - in the form of sodium ions - into compartments within the cells called vacuoles. Once the salt is stashed inside the vacuoles it is isolated from the rest of the cell and unable to interfere with the plant's normal biochemical activity.

These genetically engineered salt tolerant plants actually remove salt from the soil. Because their salt storing activity occurs only in the plants' leaves, the quality of the tomato fruit is maintained, the researchers said.

Blumwald and colleagues have demonstrated that the genetically engineered tomato plants grow and produce fruit even in irrigation water that is about 50 times saltier than normal. The plants were irrigated with water more than one third as salty as seawater.

The tomato discovery is a continuation of Blumwald's research on salt tolerance in plants. In 1999 Blumwald and his colleagues announced that they had discovered the gene that governs production of the transport protein and genetically engineered salt tolerance in the Arabidopsis plant.

The transgenic tomato plants were grown in greenhouses at the University of Toronto. Blumwald hopes to continue the research at UC Davis, including field trials in salt damaged soils. He projects that, with proper funding, it would be possible to develop commercially useful salt tolerant tomato plants within three years.

But would the engineered tomatoes find public acceptance? In June, an ABCNews.com poll conducted in the United States found that 52 percent of respondents believed that engineered foods are "not safe to eat."

Just 35 percent of respondents expressed confidence in engineered foods. In contrast, a U.S. Gallup poll conducted in summer 2000 showed that 51 percent of respondents believed that engineered foods posed no health hazard.